Hotspot

trnL-UAG-rpl32

SSC · 124053-124865 · 813 bp

Back to Pseudognaphalium

Pi 0.0255
MarkerSeek score 76.10
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
813
variable_sites
43
pi
0.0255
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 926-955 bp 1.0 85.7
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 927-956 bp 1.0 85.7
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 937-966 bp 1.0 85.5
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 938-967 bp 1.0 85.5
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 57.9 57.9 936-965 bp 1.0 84.7