Hotspot

ndhD-ccsA

SSC · 122635-122905 · 271 bp

Back to Pseudognaphalium

Pi 0.00959

MarkerSeek score 59.80

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 271
variable_sites: 5
pi: 0.00959
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCTT	42.9	50.0	57.4	58.7	421-431 bp	1.0	63.2
ndhD-ccsA_p2	TCAGTTGACAAGGTTGAAGGT	CAATTGTGGCGACTCTAGGC	42.9	55.0	57.4	59.0	427-437 bp	1.0	61.1
ndhD-ccsA_p3	TCAGTTGACAAGGTTGAAGGT	GCAATTGTGGCGACTCTAGG	42.9	55.0	57.4	59.0	428-438 bp	1.0	61.1
ndhD-ccsA_p4	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCT	42.9	52.6	57.4	58.0	421-431 bp	1.0	60.9
ndhD-ccsA_p5	TCAGTTGACAAGGTTGAAGGT	GTGGCGACTCTAGGCTTTC	42.9	57.9	57.4	57.9	422-432 bp	1.0	60.7