Hotspot

ndhA-ndhI

SSC · 118541-118552 · 12 bp

Back to Pseudognaphalium

Pi 0.0333

MarkerSeek score 60.50

Primer available yes

Label ndhA-ndhI

Feature Metadata

label_name: ndhA-ndhI
feature_type: igs
region: SSC
length_bp: 12
variable_sites: 1
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhI_p1	GATGGACTTTACCGAGGCTG	TCGTACTGTTTGTTGGCCAT	55.0	45.0	58.1	57.7	248 bp	1.0	77.7
ndhA-ndhI_p2	AGATGGACTTTACCGAGGCT	TCGTACTGTTTGTTGGCCAT	50.0	45.0	58.1	57.7	249 bp	1.0	77.1
ndhA-ndhI_p3	TTACCGAGGCTGAGAATGGA	TCGTACTGTTTGTTGGCCAT	50.0	45.0	58.1	57.7	240 bp	1.0	77.1
ndhA-ndhI_p4	GATGGACTTTACCGAGGCTG	CTCGTACTGTTTGTTGGCCA	55.0	50.0	58.1	58.4	249 bp	1.0	76.6
ndhA-ndhI_p5	AGATGGACTTTACCGAGGCT	CTCGTACTGTTTGTTGGCCA	50.0	50.0	58.1	58.4	250 bp	1.0	76.0