Hotspot

psbE-petL

LSC · 65427-66709 · 1283 bp

Back to Pseudognaphalium

Pi 0.0148

MarkerSeek score 64.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1283
variable_sites: 35
pi: 0.0148
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGGGGATCGATTCCGT	AGCCGTTAGTAGAAAACCGA	50.0	45.0	58.0	56.6	1321-1352 bp	1.0	73.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1375-1406 bp	1.0	72.4
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AGCCGTTAGTAGAAAACCGA	55.0	45.0	58.2	56.6	1370-1401 bp	1.0	72.1
psbE-petL_p4	CTCCTGTGCTTCCAGACATG	AGCCGTTAGTAGAAAACCGA	55.0	45.0	58.3	56.6	1367-1398 bp	1.0	71.6
psbE-petL_p5	CAAAAGGGGATCGATTCCGT	AGCCGTTAGTAGAAAACCGAA	50.0	42.9	58.0	57.3	1321-1352 bp	1.0	71.4