Hotspot

atpB-rbcL

LSC · 55457-56107 · 651 bp

Back to Pseudognaphalium

Pi 0.00614

MarkerSeek score 64.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 9
pi: 0.00614
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCAGCCCCAAAAGTAGTCAT	CACCTGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	818-824 bp	1.0	72.2
atpB-rbcL_p2	GCAGCCCCAAAAGTAGTCAT	ACCTGCTTTGAATCCAACACT	50.0	42.9	58.2	58.1	817-823 bp	1.0	72.0
atpB-rbcL_p3	GCAGCCCCAAAAGTAGTCAT	GTGGTGACATAAATCCCTCCC	50.0	52.4	58.2	58.1	779-785 bp	1.0	71.9
atpB-rbcL_p4	GCAGCCCCAAAAGTAGTCAT	TGCTTTGAATCCAACACTTGC	50.0	42.9	58.2	57.9	814-820 bp	1.0	71.6
atpB-rbcL_p5	GCAGCCCCAAAAGTAGTCAT	TGGTGACATAAATCCCTCCCT	50.0	47.6	58.2	58.1	778-784 bp	1.0	71.3