Hotspot

ndhC-trnV-UAC

LSC · 51160-52379 · 1220 bp

Back to Pseudognaphalium

Pi 0.0169

MarkerSeek score 73.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 43
pi: 0.0169
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGATGCACTCCTATGAACGT	GAGAAGGTCTACGGTTCGAG	45.0	55.0	56.3	57.2	1282-1313 bp	1.0	65.9
ndhC-trnV-UAC_p2	AGATGCACTCCTATGAACGT	CGAGAAGGTCTACGGTTCGA	45.0	55.0	56.3	58.9	1283-1314 bp	1.0	65.2
ndhC-trnV-UAC_p3	GCAGAAACATAGATGCACTCC	GAGAAGGTCTACGGTTCGAG	47.6	55.0	57.0	57.2	1292-1323 bp	1.0	63.4
ndhC-trnV-UAC_p4	AGATGCACTCCTATGAACGT	AGAAGGTCTACGGTTCGAGC	45.0	55.0	56.3	59.2	1281-1312 bp	1.0	63.0
ndhC-trnV-UAC_p5	GCAGAAACATAGATGCACTCC	CGAGAAGGTCTACGGTTCGA	47.6	55.0	57.0	58.9	1293-1324 bp	1.0	62.7