Hotspot

ycf3-trnS-GGA

LSC · 44788-45712 · 925 bp

Back to Pseudognaphalium

Pi 0.00721

MarkerSeek score 63.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 15
pi: 0.00721
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTCAA	50.0	45.0	58.1	57.8	1058-1067 bp	1.0	80.4
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	GGATTCGAACCCTCGGTAAG	50.0	55.0	58.1	57.8	1096-1105 bp	1.0	80.1
ycf3-trnS-GGA_p3	TCTCCTGAGGTTGTCGGAAT	AGTTCCAATGCTACGCCTTC	50.0	50.0	58.1	58.3	1060-1069 bp	1.0	79.8
ycf3-trnS-GGA_p4	TCTCCTGAGGTTGTCGGAAT	CCTCGGTAAGCAAAAGCCTA	50.0	50.0	58.1	57.6	1086-1095 bp	1.0	78.7
ycf3-trnS-GGA_p5	TTCTCCTGAGGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	50.0	45.0	58.3	57.8	1059-1068 bp	1.0	78.6