Hotspot

ycf1

SSC · 123410-128989 · 5580 bp

Back to Pseudogalium

Pi 0.0055

MarkerSeek score 29.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5580
variable_sites: 46
pi: 0.0055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCAACCTTTCCACGAATCC	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	58.1	58.0	1030 bp	1.0	80.6
ycf1_p2	ACCAACCTTTCCACGAATCC	ACCTCCAGGAATGGAATCGA	50.0	50.0	58.1	58.1	1266 bp	1.0	79.5
ycf1_p3	ACCAACCTTTCCACGAATCC	TTTTGTCTGAAACCCTGGCA	50.0	45.0	58.1	57.8	1144 bp	1.0	79.4
ycf1_p4	ACCAACCTTTCCACGAATCC	GACCTCCAGGAATGGAATCG	50.0	55.0	58.1	57.8	1267 bp	1.0	78.5
ycf1_p5	ACCAACCTTTCCACGAATCC	AACCTGCCTTTTGGTTCTCC	50.0	50.0	58.1	58.3	1036 bp	1.0	78.3