Hotspot

ndhA

SSC · 119218-121475 · 2258 bp

Back to Pseudogalium

Pi 0.00413

MarkerSeek score 60.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2258
variable_sites: 14
pi: 0.00413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGGTTACCATAGAAAACATGTCG	CCACCAGGATTTATCAATTTGCA	43.5	39.1	57.9	57.7	2478-2487 bp	1.0	40.6
ndhA_p2	CGGTTACCATAGAAAACATGTCG	TCCACCAGGATTTATCAATTTGC	43.5	39.1	57.9	57.4	2479-2488 bp	1.0	40.6
ndhA_p3	CGGTTACCATAGAAAACATGTCG	TCATTATGGGAGAAGTTGATCGT	43.5	39.1	57.9	57.4	2387-2396 bp	1.0	40.6
ndhA_p4	ACCATAGAAAACATGTCGTGA	CCACCAGGATTTATCAATTTGCA	38.1	39.1	55.3	57.7	2473-2482 bp	1.0	40.6
ndhA_p5	CGGTTACCATAGAAAACATGTCG	TGGGAGAAGTTGATCGTTAA	43.5	40.0	57.9	54.0	2381-2390 bp	1.0	40.6