Hotspot

rpl32-trnL-UAG

SSC · 113109-113765 · 657 bp

Back to Pseudogalium

Pi 0.0101

MarkerSeek score 51.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 657
variable_sites: 10
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGGGGGATATTGGACAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.2	57.9	793-795 bp	1.0	74.3
rpl32-trnL-UAG_p2	AAAGGGGGATATTGGACAGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.2	57.8	786-788 bp	1.0	73.8
rpl32-trnL-UAG_p3	AAAGGGGGATATTGGACAGC	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.2	57.8	792-794 bp	1.0	73.7
rpl32-trnL-UAG_p4	AAAGGGGGATATTGGACAGC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.2	57.8	791-793 bp	1.0	73.7
rpl32-trnL-UAG_p5	AAAGGGGGATATTGGACAGC	GAGCAGCGTGTCTACCAATT	50.0	50.0	57.2	58.3	787-789 bp	1.0	73.1