Hotspot

rps12-clpP

LSC · 69651-69771 · 121 bp

Back to Pseudogalium

Pi 0.011

MarkerSeek score 51.00

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 2
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CACCCGAGTACATGTTCCTC	TGTCGATCTTGTAGCGGTTG	55.0	50.0	57.7	58.0	230-257 bp	1.0	82.2
rps12-clpP_p2	CACCCGAGTACATGTTCCTC	ATGTCTGCAACAGAAGCACA	55.0	45.0	57.7	58.0	262-289 bp	1.0	82.0
rps12-clpP_p3	CACCCGAGTACATGTTCCTC	TCTGCAACAGAAGCACAAGT	55.0	45.0	57.7	58.0	259-286 bp	1.0	81.9
rps12-clpP_p4	ACACCCGAGTACATGTTCCT	TGTCGATCTTGTAGCGGTTG	50.0	50.0	58.4	58.0	231-258 bp	1.0	81.7
rps12-clpP_p5	ACACCCGAGTACATGTTCCT	ATGTCTGCAACAGAAGCACA	50.0	45.0	58.4	58.0	263-290 bp	1.0	81.5