Hotspot

ycf4-cemA

LSC · 60395-61231 · 837 bp

Back to Pseudogalium

Pi 0.00239

MarkerSeek score 60.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 3
pi: 0.00239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	CCACCACTTAGTGACCCAAG	45.0	55.0	58.1	57.8	990-993 bp	1.0	78.2
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	TCCACCACTTAGTGACCCAA	45.0	50.0	58.1	58.2	991-994 bp	1.0	78.2
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	TTCCACCACTTAGTGACCCA	45.0	50.0	58.1	58.2	992-995 bp	1.0	78.2
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	GACCCAAGATTCCAGGCTTT	45.0	50.0	58.1	57.8	978-981 bp	1.0	77.9
ycf4-cemA_p5	CAAAAGGCTGCTGAATTGGC	CCACCACTTAGTGACCCAAG	50.0	55.0	58.6	57.8	1005-1008 bp	1.0	74.5