Hotspot

rbcL-accD

LSC · 56455-57092 · 638 bp

Back to Pseudogalium

Pi 0.0094

MarkerSeek score 54.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 9
pi: 0.0094
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACATTTGTTTTTGAGGCAATGGA	TCCATAGCGTTTTCTTGCCT	34.8	45.0	58.0	57.5	736-741 bp	1.0	53.3
rbcL-accD_p2	CACATTTGTTTTTGAGGCAATGG	TCCATAGCGTTTTCTTGCCT	39.1	45.0	57.9	57.5	737-742 bp	1.0	52.8
rbcL-accD_p3	ACATTTGTTTTTGAGGCAATGGA	AGCGTTTTCTTGCCTCCTAT	34.8	45.0	58.0	57.2	731-736 bp	1.0	50.9
rbcL-accD_p4	ACATTTGTTTTTGAGGCAATGGA	ATAGCGTTTTCTTGCCTCCT	34.8	45.0	58.0	57.2	733-738 bp	1.0	50.9
rbcL-accD_p5	ACATTTGTTTTTGAGGCAATGGA	CATAGCGTTTTCTTGCCTCC	34.8	50.0	58.0	57.2	734-739 bp	1.0	50.6