Pi
0.00849
MarkerSeek score
64.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 978-1001 bp | 1.0 | 82.8 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 986-1009 bp | 1.0 | 82.2 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | GTTTCCATACCAAGGCCCAA | 55.0 | 50.0 | 57.9 | 58.1 | 1020-1043 bp | 1.0 | 82.1 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1010-1033 bp | 1.0 | 81.8 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | CATACCAAGGCCCAATCCAA | 55.0 | 50.0 | 57.9 | 57.8 | 1015-1038 bp | 1.0 | 81.4 |