Hotspot

trnT-GGU-psbD

LSC · 31814-33212 · 1399 bp

Back to Pseudogalium

Pi 0.00381

MarkerSeek score 50.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1399
variable_sites: 8
pi: 0.00381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1559-1592 bp	1.0	79.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1562-1595 bp	1.0	79.7
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACGGTCTCTACGTAACCAGT	55.0	50.0	58.1	57.8	1538-1571 bp	1.0	79.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.1	57.8	1541-1574 bp	1.0	79.1
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1536-1569 bp	1.0	78.1