Hotspot

trnE-UUC-trnT-GGU

LSC · 30934-31741 · 808 bp

Back to Pseudogalium

Pi 0.00825
MarkerSeek score 51.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
808
variable_sites
10
pi
0.00825
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTAGTATCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.1 58.0 946-974 bp 1.0 80.2
trnE-UUC-trnT-GGU_p2 CGTAGTATCCTACCCCCAGG CCATGGCGTTACTCTACCAC 60.0 55.0 58.1 58.1 925-953 bp 1.0 79.7
trnE-UUC-trnT-GGU_p3 CGTAGTATCCTACCCCCAGG TGGCGTTACTCTACCACTGA 60.0 50.0 58.1 58.1 922-950 bp 1.0 79.5
trnE-UUC-trnT-GGU_p4 CGTAGTATCCTACCCCCAGG AATCAGAGCCCCTTATCGGA 60.0 50.0 58.1 57.9 969-997 bp 1.0 79.4
trnE-UUC-trnT-GGU_p5 CGTAGTATCCTACCCCCAGG TCAGAGCCCCTTATCGGATT 60.0 50.0 58.1 57.9 967-995 bp 1.0 79.4