Hotspot

ycf15-trnC-GCA

LSC · 27319-28353 · 1035 bp

Back to Pseudogalium

Pi 0.0161

MarkerSeek score 57.80

Primer available yes

Label ycf15-trnC-GCA

Feature Metadata

label_name: ycf15-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1035
variable_sites: 25
pi: 0.0161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnC-GCA_p1	TGTTCTTGCATATCCCTGCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1367-1387 bp	1.0	81.9
ycf15-trnC-GCA_p2	CCGGGGCTTTGCAATATTTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1469-1489 bp	1.0	81.7
ycf15-trnC-GCA_p3	TACCGGATCTCCACCTACAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1791-1811 bp	1.0	81.1
ycf15-trnC-GCA_p4	TTCCCTCATTTCCATCCCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1122 bp	0.666667	66.1
ycf15-trnC-GCA_p5	TTCCCTCATTTCCATCCCCT	GCCAAGAGAATCCCGAAGTT	50.0	50.0	58.0	57.9	1330 bp	0.666667	65.8