Hotspot

rps2-rpoC2

LSC · 16730-16969 · 240 bp

Back to Pseudogalium

Pi 0
MarkerSeek score 56.80
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
240
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CCGCCTCCATCATTTCTTCT TTCGAGAGGGAGATGAGAGA 50.0 50.0 57.7 56.6 393-400 bp 1.0 67.8
rps2-rpoC2_p2 CCGCCTCCATCATTTCTTCT TCGAGAGGGAGATGAGAGATC 50.0 52.4 57.7 57.6 392-399 bp 1.0 67.8
rps2-rpoC2_p3 CCGCCTCCATCATTTCTTCT CGAGAGGGAGATGAGAGATCT 50.0 52.4 57.7 57.6 391-398 bp 1.0 67.7
rps2-rpoC2_p4 CCGCCTCCATCATTTCTTCT TCGAGAGGGAGATGAGAGAT 50.0 50.0 57.7 56.4 392-399 bp 1.0 66.0
rps2-rpoC2_p5 CCCGCCTCCATCATTTCTTC TTCGAGAGGGAGATGAGAGA 55.0 50.0 58.7 56.6 394-401 bp 1.0 65.1