Hotspot

trnS-GCU-trnG-UCC

LSC · 8781-9370 · 590 bp

Back to Pseudogalium

Pi 0.0113
MarkerSeek score 44.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
590
variable_sites
10
pi
0.0113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AAACCGAAAGCAAAGACCCT 50.0 45.0 58.0 57.9 702-706 bp 1.0 80.7
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA AACCGAAAGCAAAGACCCTT 50.0 45.0 58.0 57.9 701-705 bp 1.0 80.7
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACCGAAAGCAAAGACCCTTT 50.0 45.0 58.0 57.9 700-704 bp 1.0 80.7
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AACCGAAAGCAAAGACCCTT 50.0 45.0 58.0 57.9 700-704 bp 1.0 80.7
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC ACCGAAAGCAAAGACCCTTT 50.0 45.0 58.0 57.9 699-703 bp 1.0 80.7