Hotspot

trnH-GUG-psbA

LSC · 87-312 · 226 bp

Back to Pseudogalium

Pi 0.0177

MarkerSeek score 51.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 6
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CGTGCGAATCTTGGTATGGA	55.0	50.0	58.1	58.1	342-343 bp	1.0	80.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CGTGCGAATCTTGGTATGGA	50.0	50.0	57.9	58.1	356-357 bp	1.0	80.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CGTGCGAATCTTGGTATGGA	50.0	50.0	57.9	58.1	355-356 bp	1.0	80.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CGTGCGAATCTTGGTATGGA	50.0	50.0	57.9	58.1	357-358 bp	1.0	80.0
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TTAACCGTGCGAATCTTGGT	55.0	45.0	58.1	57.8	347-348 bp	1.0	79.2