Hotspot

trnK-UUU

LSC · 4179-4215 · 37 bp

Back to Pseudodissochaeta

Pi 0.027

MarkerSeek score 65.30

Primer available yes

Label trnK-UUU

Feature Metadata

label_name: trnK-UUU
feature_type: tRNA
region: LSC
length_bp: 37
variable_sites: 1
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	CCTTTCAGGATCAGTCGTGG	CAACCAAACGTTGCCAATTT	55.0	40.0	58.0	56.2	159 bp	1.0	66.2
trnK-UUU_p2	TCAGGATCAGTCGTGGTCTT	CAACCAAACGTTGCCAATTT	50.0	40.0	58.1	56.2	155 bp	1.0	65.8
trnK-UUU_p3	TTCAGGATCAGTCGTGGTCT	CAACCAAACGTTGCCAATTT	50.0	40.0	58.1	56.2	156 bp	1.0	65.8
trnK-UUU_p4	CCTTTCAGGATCAGTCGTGG	CAACCAAACGTTGCCAATTTT	55.0	38.1	58.0	57.0	159 bp	1.0	64.2
trnK-UUU_p5	TCAGGATCAGTCGTGGTCTT	CAACCAAACGTTGCCAATTTT	50.0	38.1	58.1	57.0	155 bp	1.0	63.7