Hotspot

rpl22

LSC · 85750-86163 · 414 bp

Back to Pseudodissochaeta

Pi 0.0121

MarkerSeek score 48.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 414
variable_sites: 5
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTGTCCCATAATCCCCCACT	GTTGGACAAGTGGGGAATGT	50.0	50.0	58.0	58.0	1205-1206 bp	1.0	80.5
rpl22_p2	TTGTCCCATAATCCCCCACT	TGGACAAGTGGGGAATGTTG	50.0	50.0	58.0	58.0	1203-1204 bp	1.0	80.5
rpl22_p3	TTGTCCCATAATCCCCCACT	CGTCGCCGTAGTAAATAGGG	50.0	55.0	58.0	58.0	965-966 bp	1.0	80.4
rpl22_p4	TCTATGCGTGCAATTCCCTC	GTTGGACAAGTGGGGAATGT	50.0	50.0	58.0	58.0	1387-1388 bp	1.0	80.1
rpl22_p5	TCTATGCGTGCAATTCCCTC	TGGACAAGTGGGGAATGTTG	50.0	50.0	58.0	58.0	1385-1386 bp	1.0	80.1