Hotspot

ndhJ

LSC · 50700-51176 · 477 bp

Back to Pseudodissochaeta

Pi 0.00629

MarkerSeek score 45.00

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: LSC
length_bp: 477
variable_sites: 3
pi: 0.00629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	GAGAGACCCAACCAAGCAAA	TACCGTTCGTGGAGTCGATA	50.0	50.0	58.0	58.0	1375-1404 bp	1.0	80.2
ndhJ_p2	ATATGTGCGCGGTACAAAGT	TACCGTTCGTGGAGTCGATA	45.0	50.0	58.0	58.0	1218 bp	1.0	80.1
ndhJ_p3	ACCCAACCAAGCAAAAGTCT	TACCGTTCGTGGAGTCGATA	45.0	50.0	57.8	58.0	1370-1399 bp	1.0	79.1
ndhJ_p4	AGACCCAACCAAGCAAAAGT	TACCGTTCGTGGAGTCGATA	45.0	50.0	57.8	58.0	1372-1401 bp	1.0	79.1
ndhJ_p5	ATATGTGCGCGGTACAAAGT	CCGTTCGTGGAGTCGATAAG	45.0	55.0	58.0	58.2	1216 bp	1.0	78.9