Hotspot

ndhF-rpl32

SSC · 115323-116149 · 827 bp

Back to Pseudodissochaeta

Pi 0.0157
MarkerSeek score 67.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
827
variable_sites
13
pi
0.0157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCATTGGAAAAACGA TGAAAAAGCCTTCAACGCTG 40.0 45.0 56.7 57.5 1025-1079 bp 1.0 67.0
ndhF-rpl32_p2 TGTTGCCATTGGAAAAACGA GAAAAAGCCTTCAACGCTGT 40.0 45.0 56.7 57.5 1024-1078 bp 1.0 67.0
ndhF-rpl32_p3 TTGTTGCCATTGGAAAAACGA TGAAAAAGCCTTCAACGCTG 38.1 45.0 57.5 57.5 1026-1080 bp 1.0 64.8
ndhF-rpl32_p4 TTGTTGCCATTGGAAAAACGA GAAAAAGCCTTCAACGCTGT 38.1 45.0 57.5 57.5 1025-1079 bp 1.0 64.8
ndhF-rpl32_p5 TGTTGCCATTGGAAAAACGAT TGAAAAAGCCTTCAACGCTG 38.1 45.0 57.3 57.5 1025-1079 bp 1.0 63.1