Hotspot

petB-petD

LSC · 78440-79457 · 1018 bp

Back to Pseudodissochaeta

Pi 0.00504

MarkerSeek score 64.10

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 1018
variable_sites: 5
pi: 0.00504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	AAGCAAGGTATTTCGGGTCC	CCATGCGGGTTCTCCATAAT	50.0	50.0	57.9	57.7	1115-1135 bp	1.0	79.0
petB-petD_p2	GCAAGGTATTTCGGGTCCTT	CCATGCGGGTTCTCCATAAT	50.0	50.0	57.9	57.7	1113-1133 bp	1.0	79.0
petB-petD_p3	AGCAAGGTATTTCGGGTCCT	CCATGCGGGTTCTCCATAAT	50.0	50.0	58.7	57.7	1114-1134 bp	1.0	74.3
petB-petD_p4	AAGCAAGGTATTTCGGGTCC	GGCCCATCCCCTTAGCTAAT	50.0	55.0	57.9	58.9	1089-1109 bp	1.0	73.9
petB-petD_p5	GCAAGGTATTTCGGGTCCTT	GGCCCATCCCCTTAGCTAAT	50.0	55.0	57.9	58.9	1087-1107 bp	1.0	73.9