Hotspot

petA-psbJ

LSC · 64514-65398 · 885 bp

Back to Pseudodissochaeta

Pi 0.0148

MarkerSeek score 68.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 11
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.6	58.1	897-1025 bp	1.0	80.8
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.6	58.2	901-1029 bp	1.0	79.6
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	TTCATACTCAGGGTTGGGCT	45.0	50.0	57.6	58.3	900-1028 bp	1.0	79.0
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	GGTTCATACTCAGGGTTGGG	45.0	55.0	57.6	57.6	902-1030 bp	1.0	78.2
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	ATACTCAGGGTTGGGCTCAT	50.0	50.0	57.2	58.1	903-1031 bp	1.0	78.1