Hotspot

accD-psaI

LSC · 60411-61154 · 744 bp

Back to Pseudodissochaeta

Pi 0.0202

MarkerSeek score 67.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 15
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	872 bp	1.0	67.6
accD-psaI_p2	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	871 bp	1.0	67.6
accD-psaI_p3	AAAGGGTGTTCTGAGTGAGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	878 bp	1.0	66.9
accD-psaI_p4	AAGGGTGTTCTGAGTGAGTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	877 bp	1.0	66.9
accD-psaI_p5	AAAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.1	58.0	872 bp	1.0	65.7