Hotspot

ndhK-ndhC

LSC · 52008-52068 · 61 bp

Back to Pseudodissochaeta

Pi 0.0345

MarkerSeek score 76.90

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 2
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TTTGGGTTGTTCGAGCAAGT	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.2	58.1	133-136 bp	1.0	80.4
ndhK-ndhC_p2	TGGGTTGTTCGAGCAAGTAA	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.4	58.1	131-134 bp	1.0	77.4
ndhK-ndhC_p3	TTGGGTTGTTCGAGCAAGTA	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.4	58.1	132-135 bp	1.0	77.3
ndhK-ndhC_p4	TTTTGGGTTGTTCGAGCAAG	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.1	58.1	134-137 bp	1.0	75.4
ndhK-ndhC_p5	TGGGTTGTTCGAGCAAGTAAA	CATGGCGAAAAGGAGCATTG	42.9	50.0	58.1	58.1	131-134 bp	1.0	73.7