Hotspot

rpoB-trnC-GCA

LSC · 27457-28925 · 1469 bp

Back to Pseudodissochaeta

Pi 0.0165

MarkerSeek score 61.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1469
variable_sites: 24
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCACTTCCATCCACCAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	59.0	58.4	1533-1536 bp	1.0	71.1
rpoB-trnC-GCA_p2	TTCCCTCACTTCCATCCACC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	59.0	58.4	1538-1541 bp	1.0	71.1
rpoB-trnC-GCA_p3	GACATTCCCTCACTTCCATCC	AAAAGGATTTGCAGTCCCCC	52.4	50.0	58.1	58.4	1542-1545 bp	1.0	70.6
rpoB-trnC-GCA_p4	CATTCCCTCACTTCCATCCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	56.9	58.4	1540-1543 bp	1.0	70.3
rpoB-trnC-GCA_p5	ATTCCCTCACTTCCATCCAC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	56.9	58.4	1539-1542 bp	1.0	70.3