Hotspot

trnS-GCU-trnR-UCU

LSC · 8111-9946 · 1836 bp

Back to Pseudodissochaeta

Pi 0.00941

MarkerSeek score 57.00

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1836
variable_sites: 17
pi: 0.00941
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ACGGAAAGAGAGGGATTCGA	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.2	56.6	2004-2011 bp	1.0	69.1
trnS-GCU-trnR-UCU_p2	TAGCAATCCGACGCTTTAGG	AGAACTTGCGTCCAATAGGA	50.0	45.0	57.8	56.6	1953-1960 bp	1.0	68.6
trnS-GCU-trnR-UCU_p3	ACGGAAAGAGAGGGATTCGA	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.2	57.3	2011-2018 bp	1.0	67.2
trnS-GCU-trnR-UCU_p4	ACGGAAAGAGAGGGATTCGA	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.2	57.3	2005-2012 bp	1.0	67.1
trnS-GCU-trnR-UCU_p5	TAGCAATCCGACGCTTTAGG	ACATAGAAGAACTTGCGTCCA	50.0	42.9	57.8	57.3	1960-1967 bp	1.0	66.7