Hotspot

ycf4

LSC · 60194-60835 · 642 bp

Back to Pseudocodon

Pi 0.00156

MarkerSeek score 59.60

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 642
variable_sites: 1
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	AATGGGGTCATACGAAGGAT	TAAGCCTGCTTTAGCTCGTG	45.0	50.0	56.0	58.0	852 bp	1.0	63.8
ycf4_p2	AATGGGGTCATACGAAGGAT	GTTCGGACGAAACCTCAGTT	45.0	50.0	56.0	58.1	833 bp	1.0	62.8
ycf4_p3	TGGGGTCATACGAAGGATATG	TAAGCCTGCTTTAGCTCGTG	47.6	50.0	56.7	58.0	850 bp	1.0	61.4
ycf4_p4	GGGGTCATACGAAGGATATGT	TAAGCCTGCTTTAGCTCGTG	47.6	50.0	56.7	58.0	849 bp	1.0	61.4
ycf4_p5	AATGGGGTCATACGAAGGAT	TCCCCTCCTGCTGAGAAAAT	45.0	50.0	56.0	58.3	765 bp	1.0	61.3