Hotspot

rps14

LSC · 76463-76765 · 303 bp

Back to Pseudocodon

Pi 0.0033

MarkerSeek score 54.50

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: LSC
length_bp: 303
variable_sites: 1
pi: 0.0033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	GCGCCTCTCTACCATTCTG	AGAGTCCGTTTTGTGATTTTGC	57.9	40.9	57.7	58.0	524 bp	1.0	53.6
rps14_p2	GCGCCTCTCTACCATTCTG	GAGTCCGTTTTGTGATTTTGCT	57.9	40.9	57.7	58.0	523 bp	1.0	53.6
rps14_p3	GCGCCTCTCTACCATTCTG	AGTCCGTTTTGTGATTTTGCT	57.9	38.1	57.7	56.9	522 bp	1.0	53.3
rps14_p4	CGCCTCTCTACCATTCTGTAC	AGAGTCCGTTTTGTGATTTTGC	52.4	40.9	57.6	58.0	523 bp	1.0	53.0
rps14_p5	CGCCTCTCTACCATTCTGTAC	GAGTCCGTTTTGTGATTTTGCT	52.4	40.9	57.6	58.0	522 bp	1.0	53.0