Hotspot

psaC

SSC · 144525-144770 · 246 bp

Back to Pseudocodon

Pi 0.00407

MarkerSeek score 54.80

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GGGCCATAAAACCCATACGT	TCTGGAAACTCATCGATTCGT	50.0	42.9	57.9	57.1	447 bp	1.0	64.7
psaC_p2	GGGCCATAAAACCCATACGT	TGGAAACTCATCGATTCGTCT	50.0	42.9	57.9	57.1	445 bp	1.0	64.7
psaC_p3	GGGCCATAAAACCCATACGT	CTGGAAACTCATCGATTCGTC	50.0	47.6	57.9	56.8	446 bp	1.0	62.2
psaC_p4	GGGCCATAAAACCCATACGT	CTCTGGAAACTCATCGATTCGT	50.0	45.5	57.9	58.2	448 bp	1.0	61.9
psaC_p5	GGGCCATAAAACCCATACGT	CTGGAAACTCATCGATTCGTCT	50.0	45.5	57.9	58.2	446 bp	1.0	61.9