Hotspot

clpP-trnL-UAA

LSC · 84844-87412 · 2569 bp

Back to Pseudocodon

Pi 0.000389

MarkerSeek score 56.70

Primer available yes

Label clpP-trnL-UAA

Feature Metadata

label_name: clpP-trnL-UAA
feature_type: igs
region: LSC
length_bp: 2569
variable_sites: 1
pi: 0.000389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-trnL-UAA_p1	TCAGGATGAGGACGAGAAGA	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.2	58.1	2662 bp	1.0	73.0
clpP-trnL-UAA_p2	TCAGGATGAGGACGAGAAGA	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.2	58.2	2668 bp	1.0	71.6
clpP-trnL-UAA_p3	TCAGGATGAGGACGAGAAGA	GTACGTTTCCCTACCAAGGC	50.0	55.0	57.2	58.3	2700 bp	1.0	71.4
clpP-trnL-UAA_p4	TCAGGATGAGGACGAGAAGAA	TAGCGTCTACCAATTTCGCC	47.6	50.0	57.9	58.1	2662 bp	1.0	70.8
clpP-trnL-UAA_p5	TCAGGATGAGGACGAGAAGA	TACCAATTTCGCCATACCCC	50.0	50.0	57.2	57.6	2655 bp	1.0	70.7