Hotspot

trnM-CAU-trnfM-CAU

LSC · 74939-76219 · 1281 bp

Back to Pseudocodon

Pi 0.000781
MarkerSeek score 59.20
Primer available yes
Label trnM-CAU-trnfM-CAU

Feature Metadata

label_name
trnM-CAU-trnfM-CAU
feature_type
igs
region
LSC
length_bp
1281
variable_sites
1
pi
0.000781
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-trnfM-CAU_p1 GGATGGGTCCCATTTGGTTT TCATCGGTTCAAATCCGGTC 50.0 50.0 58.0 58.0 1418 bp 0.5 59.5
trnM-CAU-trnfM-CAU_p2 GGATGGGTCCCATTTGGTTT CATCGGTTCAAATCCGGTCT 50.0 50.0 58.0 58.0 1417 bp 0.5 59.4
trnM-CAU-trnfM-CAU_p3 GGATGGGTCCCATTTGGTTT TGGTAGCTCGTAAGGCTCAT 50.0 50.0 58.0 58.2 1448 bp 0.5 57.8
trnM-CAU-trnfM-CAU_p4 GGATGGGTCCCATTTGGTTT TCTTCTCTTAAGGGCGGAGT 50.0 50.0 58.0 57.8 1477 bp 0.5 57.7
trnM-CAU-trnfM-CAU_p5 GGATGGGTCCCATTTGGTTT GTCATCGGTTCAAATCCGGT 50.0 50.0 58.0 58.3 1419 bp 0.5 57.5