Hotspot

atpI-atpH

LSC · 42952-45223 · 2272 bp

Back to Pseudocodon

Pi 0.0022

MarkerSeek score 58.40

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 2272
variable_sites: 5
pi: 0.0022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTGCAACTTTAGCTGCGG	ATAACGGAAGCGGCAGAAAT	45.0	45.0	57.9	58.0	2359 bp	1.0	78.8
atpI-atpH_p2	TTTTGCAACTTTAGCTGCGG	TACCTTGACCAACTCCAGGT	45.0	50.0	57.9	57.9	2415 bp	1.0	78.2
atpI-atpH_p3	TTTTGCAACTTTAGCTGCGG	AGCCAATCCAGCAGCAATAA	45.0	45.0	57.9	57.9	2375 bp	1.0	77.9
atpI-atpH_p4	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.3	58.0	2408 bp	1.0	77.8
atpI-atpH_p5	TTTTGCAACTTTAGCTGCGG	AATAACGGAAGCGGCAGAAA	45.0	45.0	57.9	58.2	2360 bp	1.0	77.6