Hotspot

psbA-trnQ-UUG

LSC · 1517-2760 · 1244 bp

Back to Pseudocodon

Pi NA

MarkerSeek score 46.20

Primer available yes

Label psbA-trnQ-UUG

Feature Metadata

label_name: psbA-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1244
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnQ-UUG_p1	CGGTTTTCAGGGCTGGTTAT	TTGAACCCGGCATATCGAAG	50.0	50.0	58.2	58.1	1383 bp	0.5	58.3
psbA-trnQ-UUG_p2	ACCATCCAATGTAAAGGCGG	TTGAACCCGGCATATCGAAG	50.0	50.0	58.2	58.1	1400 bp	0.5	57.7
psbA-trnQ-UUG_p3	CCATCCAATGTAAAGGCGGT	TTGAACCCGGCATATCGAAG	50.0	50.0	58.2	58.1	1399 bp	0.5	57.7
psbA-trnQ-UUG_p4	AAGCGACCCCATAAGCTTTC	TTGAACCCGGCATATCGAAG	50.0	50.0	58.2	58.1	1353 bp	0.5	57.6
psbA-trnQ-UUG_p5	CGGTTTTCAGGGCTGGTTAT	CCTGTTGCTACAAACGCATC	50.0	50.0	58.2	57.8	1447 bp	0.5	56.7