Hotspot

sufB

LSC · 120667-122127 · 1461 bp

Back to Pseudo-nitzschia

Pi 0.0984

MarkerSeek score 80.20

Primer available yes

Label sufB

Feature Metadata

label_name: sufB
feature_type: gene
region: LSC
length_bp: 1461
variable_sites: 274
pi: 0.0984
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
sufB_p1	TCACCACCAGAAAAGCCTTC	TCTGTCGGTTGATCGTTTGT	50.0	45.0	58.0	57.5	2175 bp	0.2	43.7
sufB_p2	AGGCGACATGTTCACAAGTT	TCTGTCGGTTGATCGTTTGT	45.0	45.0	58.0	57.5	2131 bp	0.2	43.6
sufB_p3	GGATGTCCGGCTAAAACCTT	TCTGTCGGTTGATCGTTTGT	50.0	45.0	57.9	57.5	1878 bp	0.2	42.9
sufB_p4	CACCACCAGAAAAGCCTTCA	TCTGTCGGTTGATCGTTTGT	50.0	45.0	58.3	57.5	2174 bp	0.2	41.4
sufB_p5	TCACCACCAGAAAAGCCTTC	AGTTCTGTCGGTTGATCGTT	50.0	45.0	58.0	57.2	2178 bp	0.2	41.3