Hotspot

trnS(gct)-trnD(gtc)

LSC · 105191-105271 · 81 bp

Back to Pseudo-nitzschia

Pi 0.291

MarkerSeek score 81.80

Primer available yes

Label trnS(gct)-trnD(gtc)

Feature Metadata

label_name: trnS(gct)-trnD(gtc)
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 40
pi: 0.291
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(gct)-trnD(gtc)_p1	AATGGCTGAGTGGTCGAAAG	AGACTCGAACTCGCAACTTC	50.0	50.0	58.2	57.9	213-226 bp	1.0	90.1
trnS(gct)-trnD(gtc)_p2	AATGGCTGAGTGGTCGAAAG	GAGACTCGAACTCGCAACTT	50.0	50.0	58.2	57.9	214-227 bp	1.0	90.1
trnS(gct)-trnD(gtc)_p3	GAAATGGCTGAGTGGTCGAA	AGACTCGAACTCGCAACTTC	50.0	50.0	58.2	57.9	215-228 bp	1.0	90.0
trnS(gct)-trnD(gtc)_p4	GAAATGGCTGAGTGGTCGAA	GAGACTCGAACTCGCAACTT	50.0	50.0	58.2	57.9	216-229 bp	1.0	90.0
trnS(gct)-trnD(gtc)_p5	GGAGAAATGGCTGAGTGGTC	AGACTCGAACTCGCAACTTC	55.0	50.0	58.3	57.9	218-231 bp	1.0	89.5