Hotspot

trnM(cat)-trnR(ccg)

LSC · 76033-76486 · 454 bp

Back to Pseudo-nitzschia

Pi 0.167

MarkerSeek score 82.20

Primer available yes

Label trnM(cat)-trnR(ccg)

Feature Metadata

label_name: trnM(cat)-trnR(ccg)
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 129
pi: 0.167
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM(cat)-trnR(ccg)_p1	ACCAGTGATGTTCCAGAGGA	GGTTCGATTCCCTTCATGCT	50.0	50.0	58.0	58.0	525-542 bp	0.8	82.9
trnM(cat)-trnR(ccg)_p2	ACCAGTGATGTTCCAGAGGA	AGAGAAATGGGGTGGTGGTA	50.0	50.0	58.0	57.9	699-717 bp	0.8	82.0
trnM(cat)-trnR(ccg)_p3	ACCAGTGATGTTCCAGAGGA	GTGCGGATATTTACCCACGT	50.0	50.0	58.0	58.1	790-808 bp	0.6	73.3
trnM(cat)-trnR(ccg)_p4	ACCAGTGATGTTCCAGAGGA	TGGTGCATGTCACTTAGGTG	50.0	50.0	58.0	57.8	987 bp	0.2	46.3
trnM(cat)-trnR(ccg)_p5	ACCAGTGATGTTCCAGAGGA	TGTGGTGCATGTCACTTAGG	50.0	50.0	58.0	57.8	989 bp	0.2	46.3