Hotspot

trnR(acg)-trnQ(ttg)

SSC · 57039-57077 · 39 bp

Back to Pseudo-nitzschia

Pi 0.205

MarkerSeek score 81.00

Primer available yes

Label trnR(acg)-trnQ(ttg)

Feature Metadata

label_name: trnR(acg)-trnQ(ttg)
feature_type: igs
region: SSC
length_bp: 39
variable_sites: 16
pi: 0.205
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(acg)-trnQ(ttg)_p1	TGGACTAGAGCACGTGGTTA	ATGGCAGGATTCGAACCTAC	50.0	50.0	58.1	57.4	164-166 bp	1.0	82.6
trnR(acg)-trnQ(ttg)_p2	GGGGTTCGAATCCCTTCTTG	ATGGCAGGATTCGAACCTAC	55.0	50.0	58.2	57.4	129-131 bp	1.0	81.7
trnR(acg)-trnQ(ttg)_p3	GGACTAGAGCACGTGGTTAC	ATGGCAGGATTCGAACCTAC	55.0	50.0	57.7	57.4	163-165 bp	1.0	81.1
trnR(acg)-trnQ(ttg)_p4	TGGACTAGAGCACGTGGTTA	GATGGCAGGATTCGAACCTA	50.0	50.0	58.1	57.1	165-167 bp	1.0	80.2
trnR(acg)-trnQ(ttg)_p5	TGGACTAGAGCACGTGGTTA	GGCAGGATTCGAACCTACG	50.0	57.9	58.1	58.0	162-164 bp	1.0	79.4