Hotspot

rnl-rrn5

IRb · 19036-19086 · 51 bp

Back to Pseudo-nitzschia

Pi 0.0824

MarkerSeek score 80.30

Primer available yes

Label rnl-rrn5

Feature Metadata

label_name: rnl-rrn5
feature_type: igs
region: IRb
length_bp: 51
variable_sites: 8
pi: 0.0824
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rnl-rrn5_p1	GATGTGCTAACAGACCGAGG	GTGGTTCCGCTGAATACACT	55.0	50.0	58.1	57.9	105-106 bp	1.0	85.5
rnl-rrn5_p2	GATGTGCTAACAGACCGAGG	AGTGTGGTTCCGCTGAATAC	55.0	50.0	58.1	57.9	108-109 bp	1.0	85.3
rnl-rrn5_p3	GATGTGCTAACAGACCGAGG	CAGTGTGGTTCCGCTGAATA	55.0	50.0	58.1	57.9	109-110 bp	1.0	85.2
rnl-rrn5_p4	GATGTGCTAACAGACCGAGG	TGTGGTTCCGCTGAATACAC	55.0	50.0	58.1	58.2	106-107 bp	1.0	84.6
rnl-rrn5_p5	GATGTGCTAACAGACCGAGG	TCGAGATGGATCAGTGTGGT	55.0	50.0	58.1	58.1	120-121 bp	1.0	84.2