Hotspot

rpl22

LSC · 129865-130251 · 387 bp

Back to Pseudalcantarea

Pi 0

MarkerSeek score 22.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 387
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	TCAAAAAGGTAGGGAATAACC	47.6	38.1	58.1	53.1	499-500 bp	1.0	40.0
rpl22_p2	CCAAGTCTGAAACCAAGTGGA	GACAAATTTCAAAAAGGTAGGGA	47.6	34.8	58.1	55.3	507 bp	0.5	20.0
rpl22_p3	CCAAGTCTGAAACCAAGTGGA	ACAAATTTCAAAAAGGTAGGGA	47.6	31.8	58.1	54.1	506 bp	0.5	20.0
rpl22_p4	ACCAAGTCTGAAACCAAGTGG	GACAAATTTCAAAAAGGTAGGGA	47.6	34.8	58.3	55.3	508 bp	0.5	20.0
rpl22_p5	CCAAGTCTGAAACCAAGTGGA	GACAAATTTCAAAAAGGTAGGGAA	47.6	33.3	58.1	56.0	507 bp	0.5	20.0