Hotspot

rpl16

LSC · 127523-129012 · 1490 bp

Back to Pseudalcantarea

Pi 0.00206

MarkerSeek score 61.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1490
variable_sites: 3
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCCAATACCCCTTTTTGT	AGAATTCGATTGATCATTTGGT	45.0	31.8	56.6	54.1	1539-1555 bp	1.0	41.8
rpl16_p2	TCCCCAATACCCCTTTTTGT	AAGAATTCGATTGATCATTTGGT	45.0	30.4	56.6	54.9	1540-1556 bp	1.0	41.8
rpl16_p3	TCCCCAATACCCCTTTTTGTT	AGAATTCGATTGATCATTTGGT	42.9	31.8	57.4	54.1	1539-1555 bp	1.0	41.8
rpl16_p4	TCCCCAATACCCCTTTTTGTT	AAGAATTCGATTGATCATTTGGT	42.9	30.4	57.4	54.9	1540-1556 bp	1.0	41.8
rpl16_p5	ATCCCCAATACCCCTTTTTGT	AGAATTCGATTGATCATTTGGT	42.9	31.8	57.1	54.1	1540-1556 bp	1.0	41.8