Hotspot

rrn16S-trnI-GAU

IRa · 135699-136014 · 316 bp

Back to Pseudalcantarea

Pi 0.0443

MarkerSeek score 78.30

Primer available yes

Label rrn16S-trnI-GAU

Feature Metadata

label_name: rrn16S-trnI-GAU
feature_type: igs
region: IRa
length_bp: 316
variable_sites: 14
pi: 0.0443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	410 bp	1.0	78.9
rrn16S-trnI-GAU_p2	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	442 bp	1.0	78.8
rrn16S-trnI-GAU_p3	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	55.0	50.0	57.7	57.8	461 bp	1.0	78.7
rrn16S-trnI-GAU_p4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	58.3	57.8	464 bp	1.0	78.6
rrn16S-trnI-GAU_p5	AGTGACTGGAGTGAAGTCGT	CGCTGATGAGCACATTGAAC	50.0	50.0	58.3	57.8	483 bp	1.0	78.5