Hotspot

rpl22-ndhB

LSC · 130252-130302 · 51 bp

Back to Pseudalcantarea

Pi 0.229

MarkerSeek score 92.10

Primer available yes

Label rpl22-ndhB

Feature Metadata

label_name: rpl22-ndhB
feature_type: igs
region: LSC
length_bp: 51
variable_sites: 11
pi: 0.229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 92.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-ndhB_p1	CGCCCACGAATCTGATCAAT	GCCCTATTGTTCCGATGGAG	50.0	55.0	58.4	58.1	228-231 bp	1.0	80.6
rpl22-ndhB_p2	CGCCCACGAATCTGATCAAT	ATAACGGCCCTATTGTTCCG	50.0	50.0	58.4	57.8	234-237 bp	1.0	79.4
rpl22-ndhB_p3	CATAGGAACGCCCACGAATC	GCCCTATTGTTCCGATGGAG	55.0	55.0	58.8	58.1	236-239 bp	1.0	77.5
rpl22-ndhB_p4	CGCCCACGAATCTGATCAAT	TCCGATGGAGAGAAGAACCT	50.0	50.0	58.4	57.5	218-221 bp	1.0	77.5
rpl22-ndhB_p5	ATAGGAACGCCCACGAATCT	GCCCTATTGTTCCGATGGAG	50.0	55.0	58.9	58.1	235-238 bp	1.0	76.8