Hotspot

psaJ-rpl33

LSC · 113295-113818 · 524 bp

Back to Pseudalcantarea

Pi 0.00954

MarkerSeek score 62.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 5
pi: 0.00954
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	CCCGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	683 bp	1.0	79.7
psaJ-rpl33_p2	TATTCCCAGATGCCCTGTCA	CCCGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	644 bp	1.0	79.3
psaJ-rpl33_p3	CGTGGCACCTGTGTTAACTA	CCCGCGATTCCTTATTGACA	50.0	50.0	57.8	58.1	712 bp	1.0	78.9
psaJ-rpl33_p4	ATGGTTTGGGTCTTTAGCGG	CCCGCGATTCCTTATTGACA	50.0	50.0	58.2	58.1	688 bp	1.0	78.8
psaJ-rpl33_p5	TGGGTCTTTAGCGGGTCTAT	CCCGCGATTCCTTATTGACA	50.0	50.0	57.8	58.1	682 bp	1.0	78.8