Hotspot

ycf4-cemA

LSC · 105896-106746 · 851 bp

Back to Pseudalcantarea

Pi 0.00239

MarkerSeek score 61.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 2
pi: 0.00239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	TGGGAAAGAAGGCAATGCTT	45.0	45.0	57.8	58.0	925-939 bp	1.0	80.0
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.8	57.8	971-985 bp	1.0	78.8
ycf4-cemA_p3	GCCATTCCCTTGACTCGTAC	TGGGAAAGAAGGCAATGCTT	55.0	45.0	58.3	58.0	975-989 bp	1.0	78.4
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	ATGGGAAAGAAGGCAATGCT	45.0	45.0	57.8	57.8	926-940 bp	1.0	78.2
ycf4-cemA_p5	GCCATTCCCTTGACTCGTAC	ATGAGAGAGAGACCCACCAG	55.0	55.0	58.3	57.8	1021-1035 bp	1.0	77.2