Hotspot

ndhC-trnV-UAC

LSC · 96051-97091 · 1041 bp

Back to Pseudalcantarea

Pi 0.00384

MarkerSeek score 61.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1041
variable_sites: 4
pi: 0.00384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1178 bp	1.0	78.4
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAACTCC	GAGCCCAATGCAACAAACAA	55.0	45.0	58.0	57.8	1265 bp	1.0	78.1
ndhC-trnV-UAC_p3	CGGGGCTAAAACTCCTGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1173 bp	1.0	77.1
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCTGAAA	GAGCCCAATGCAACAAACAA	50.0	45.0	57.8	57.8	1260 bp	1.0	76.8
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAACTCCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1177 bp	1.0	76.0