Hotspot

rpoB-trnC-GCA

LSC · 71717-73088 · 1372 bp

Back to Pseudalcantarea

Pi 0.00148

MarkerSeek score 61.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1372
variable_sites: 2
pi: 0.00148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.4	58.1	1427-1439 bp	1.0	77.4
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATTCCGG	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.6	58.1	1428-1440 bp	1.0	77.2
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1453-1465 bp	1.0	74.2
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	50.0	50.0	57.6	58.5	1454-1466 bp	1.0	74.0
rpoB-trnC-GCA_p5	TCTTCCGTCAAGCCTTGATT	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.1	58.1	1504-1516 bp	1.0	73.8